Overexpression Plasmid

The target coding sequence (CDS) was cloned into plasmid, and promoter on plasmid regulates the expression of the target gene. In addition, the reporter gene and selection marker can be used for screening. Ubigene developed a series of YOE plasmids, including lentivirus plasmids, AAV plasmids, and non-viral plasmids, which can be widely used in vitro or in vivo.

YOE series	Vector	Reporter gene; Selection marker
Lentiviral YOE plasmid	YOE-LV001	EGFP/mCherry; Puro/Neo
Non-viral YOE plasmid	YOE-RP001	EGFP/mCherry; Puro/Neo
AAV YOE plasmid	YOE-AVV001	EGFP/mCherry

Note：Ubigene provides customized plasmid solutions. In addition to the selection of the above-mentioned plasmid backbone, customers can also choose different promoters: constitutive/specific/inducible promoter, the selection of connection mode between multiple target genes, etc.

Validation

Restriction enzyme digestion and sequencing

Application

Different species: mammals, zebrafish, primary cells, stem cells, cell lines, microorganisms, etc.
Different purposes: frame-shift mutation knockout, fragment knockout, precise knockout, point mutation, fragment knockin.

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Overexpression Plasmid

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