Gene knockdown plasmid
RNA interference (RNAi) is induced by a highly conserved, double-stranded RNA (dsRNA), which would cause specific degradation of homologous mRNA. Specific genes can be silenced, resulting in the loss of function or suppression of gene expression.
YSH series
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Vector
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Reporter gene; Selection marker
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Lentiviral YSH plasmid
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YOE-LV001
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EGFP/mCherry; Puro/Neo
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Non-viral YSH plasmid
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YSH-RP001
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EGFP/mCherry; Puro/Neo
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AAV YSH plasmid
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YSH-AVV001
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EGFP/mCherry
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Note: Ubigene provides customized plasmid solutions, including different types of backbone, expression system, shRNA design, and Pol II promoters (Mir30-shRNA), etc.
Validation
Restriction enzyme digestion and sequencing
Application
Different species: mammals, zebrafish, primary cells, stem cells, cell lines, microorganisms, etc.
Different purposes: frame-shift mutation knockout, fragment knockout, precise knockout, point mutation, fragment knockin.